1253 The senescence of melanocytes is driven by glycolytic changes and leads to melanosome transfer dysfunction and accumulation of melanin

Senescent melanocytes accumulate in photoaged skin and are closely related to aging. A better understanding of molecular characteristics senescent might be a key controlling We have developed an vitro model senescence using UV irradiation highlighted that characterized by melanosome transfer dysfunction resulting melanin accumulation. The defective transport was confirmed with ultrastructural characterization UV-induced hypopigmented aging skin. Single cell transcriptomic analysis revealed the glycolytic metabolism pathway appeared significantly upregulated most phenotypes. Furthermore, inhibition glycolysis pharmacological compounds mitigates pro-aging effects senescence, suggesting alterations cellular glucose act as driving force for melanocytes. Taken together, these results demonstrated which changes, highlighting importance metabolic reprogramming regulating melanocyte senescence.